Sanae Nagatsuki is a girl who loves Squid Girl, and because of this Squid Girl is scared of her. Early in the series, Sanae took Squid Girl home and dressed her up in mutiple outfits. She has a collection of photos of Squid Girl. She also liked her before, Eiko thought that in the episode she debut in, she thinks Squid Girl attack her.

Sanae has short brown hair which is complimented by white head band. she has light brown eyes and a fragile figure. The outfit she is most seen in is a light blue tank top and a sky blue plaid mini skirt. When at school she wears a Japenese school girl outfit.